Hifair® iii 1st strand cdna synthesis kit
WebO Kit Explorer chega. Folhetos digitais 7.18.2024 Ofertas até 31/07/22 2.27.2024 Catálogo PeakTech 2024 1.15.2024 Controle e sinalização 2024 1.1.2016 Chauvin Arnoux/Metrix … WebProtoScript® First Strand cDNA Synthesis Kit features two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix. M-MuLV Enzyme Mix combines M-MuLV Reverse Transcriptase and Murine RNase Inhibitor, while M-MuLV Reaction Mix contains dNTPs and an optimized buffer.
Hifair® iii 1st strand cdna synthesis kit
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WebThis enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). WebIt can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity and higher yield of cDNA. The kit also provides two optimized primers for …
WebThe Invitrogen SuperScript III First-Strand Synthesis System SuperMix is an optimized SuperMix formulation for first-strand cDNA synthesis from purified poly (A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 0.1 pg to 5 μg of total RNA. WebYesen Biotech hifair iii 1st strand cdna synthesis supermix kit Hifair Iii 1st Strand Cdna Synthesis Supermix Kit, supplied by Yesen Biotech, used in various techniques. Bioz …
WebTotal colonic RNA was isolated with the help of the MolPure ® Cell/Tissue Total RNA Kit (Yeasen Biotech, Shanghai, China). cDNA was reverse transcribed using the Hifair ® III 1st Strand cDNA Synthesis kit (Yeasen Biotech, Shanghai, China) and quantified with a real-time PCR system (LightCycler 96, Roche, Germany) using qPCR SYBR Green Master Mix. WebNZY First-Strand cDNA Synthesis Kit is a convenient and reliable system that includes all the necessary components to synthesize first-strand cDNA. The kit allows reaction at …
WebProtoScript II First Strand cDNA Synthesis Kit features two optimized mixes, ProtoScript II Enzyme Mix and ProtoScript II Reaction Mix. The enzyme mix combines ProtoScript …
WebPCR Kit includes the components provided with MIRC-10, plus SYBR® GreenER™ qPCR SuperMix Universal. Number of Reactions Cat. no. Polyadenylation cDNA Synthesis qPCR MIRC-10 10 20 — MIRC-50 50 100 — MIRQ-100 10 20 100 MIRQER-100 10 20 100 NCode™ miRNA First-Strand cDNA Synthesis Kits The following reagents and … can a financial advisor help me buy a houseWeb23 de fev. de 2024 · Hifair III 1st Strand cDNA Synthesis SuperMix for qPCR and Hieff qPCR SYBR Master Mix were obtained from Yeasen Biotech Co., Ltd. PCR primers were purchased from Comate Bioscience Co., Ltd. Cell lysis buffer for Western blotting and IP, CCK-8 Kit, RIPA lysis buffer, and TNF-α ELISA kit were purchased from Beyotime … can a fingernail reattach itselfWebDetails: Product Description Hifair™ Ⅲ Reverse Transcriptase is an updated version of Hifair™ Ⅱ Reverse Transcriptase obtained through genetic engineering technology. It … can a financial advisor help with my 401kWebThe reactions described in the SuperScript™ Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50 µg total RNA, or 0.2–5 µg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this ... fisherman\u0027s house restaurantWebTotal colon RNA was extracted using a MolPure® Cell/Tissue Total RNA Kit (Yeasen Biotech, Shanghai, China). cDNA was reverse transcribed using a Hifair® III 1st Strand cDNA Synthesis kit (Yeasen Biotech, Shanghai, China). qRT-PCR was performed using SYBR Green Master Mix with samples run on a LightCycler 96 (Roche, Germany). fisherman\u0027s house on a lake by albrecht dürerWebCell Counting Kit-8 Proliferation Assay. A CCK8 kit (Lunchangshuo, Biotech Co. Ltd, China) was used to measure ... China), a CR0232 lncRNA First Strand cDNA Synthesis Kit (Cellregen,China), and Hifair ® III 1st Strand cDNA Synthesis SuperMix for qPCR (Yeasen, China) were used for miRNA reverse transcription, lncRNA reverse … can a financial advisor make you richWebThe RT² Easy First Strand Kit provides a rapid and convenient procedure for efficient first strand cDNA synthesis and genomic DNA elimination of your RNA samples. Its unique format enables easy parallel processing of multiple RNA samples for reverse transcription. The synthesized cDNA is ready to use in real-time PCR for monitoring expression of fisherman\u0027s house くろいわ