WebNov 30, 2024 · The ITS2 region of an even (i.e. having equal proportions of each species) 19-species fungal mock community provided by Matt Bakker (U.S. Department of Agriculture, Peoria, IL, US) for composition see Supplementary Table 3) was amplified using the primers F-ITS4 5-TCCTCCGCTTATTGATATGC and R-fITS7 5 … During the last 20 years, analysis of microbial communities based on amplification and sequencing of genetic markers has revolutionized fungal ecology (Horton & Bruns, 2001; Hibbett et al., 2009). High-throughput methods, such as 454-pyrosequencing (Margulies et al., 2005), enable sequencing of … See more We present three new primers, which may be used together with the ITS4 primer to amplify the ITS2 region of fungal templates. The relatively short amplicons lead to high amplification efficiency, reducing the number … See more During PCR amplification of full-length fungal ITS fragments, community composition may be severely distorted because of … See more
Floral fungal-bacterial community structure and co
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Soil fungi ITS2 region PCR acmplicons have double bands?
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